Q5® Site-Directed Mutagenesis Kit

Site Directed Mutagenesis (SDM) Mouse - C2C12 miR-145 target site

Experiment
Site Directed Mutagenesis (SDM) Mouse - C2C12 miR-145 target site
Product
Q5® Site-Directed Mutagenesis Kit from New England BioLabs
Manufacturer
New England BioLabs

Protocol tips

Protocol tips
- Only use 1 μl of PCR product in the KLD reaction. Carrying too much PCR product forward can decrease transformation efficiency.

- Only use 5 μl of the KLD reaction in the transformation. If more KLD reaction is added, a buffer exchange step, such as PCR
purification, should be included prior to transformation.

Publication protocol

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Papers

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Manufacturer protocol

Download the product protocol from New England BioLabs for Q5® Site-Directed Mutagenesis Kit below.

Download PDF Download manufacturer protocol

Videos

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