In Situ Cell Death Detection Kit, Fluorescein

TUNEL assay cell type - HNSCC Detroit 562 human head and neck tumor cells

Experiment
TUNEL assay cell type - HNSCC Detroit 562 human head and neck tumor cells
Product
In Situ Cell Death Detection Kit, Fluorescein from Sigma-Aldrich
Manufacturer
Sigma-Aldrich

Protocol tips

Upstream tips
- Cells were pre- treated with various concentrations of rigosertib for 48 hours and stained with TUNEL reaction mixture
Downstream tips
- When Nonspecific labelling is present, reduce concentration of TdT

- When high background is present reduce concentration of labelling mix by 50%

Publication protocol

Terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) was conducted using Cell Death Detection Kit, Fluorescein (Roche Diagnostics, Basel, Switzerland). Cells were treated as described above with various concentrations of rigosertib for 48 hours and stained with TUNEL reaction mixture per the manufacturer's instructions. Staining was quantified using a FACSVANTAGEā„¢ flow cytometer (BD Biosciences, San Jose, CA) or images were captured by fluorescent microscopy.

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Discussion

Discussion

3 years ago

Author: Ercole Udinesi Italy

Are 10 week old samples in formalin still usable?

I have left my tumor samples in formalin for an extended period of time (around 10 weeks). Do you think I will be able to use them for TUNEL assay and get results? Thank you for your help in advance

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Papers

Check out relevant papers found by Labettor's AI that are relevant for performing TUNEL assay cell type - HNSCC Detroit 562 human head and neck tumor cells using In Situ Cell Death Detection Kit, Fluorescein from Sigma-Aldrich.

Paper title
ON 01910.Na (rigosertib) inhibits PI3K/Akt pathway and activates oxidative stress signals in head and neck cancer cell lines
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Manufacturer protocol

Download the product protocol from Sigma-Aldrich for In Situ Cell Death Detection Kit, Fluorescein below.

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