QuikChange Site-Directed Mutagenesis Kit, 10 Rxn

Site Directed Mutagenesis (SDM) Rat - Point mutation H9C2 CELF1

Experiment
Site Directed Mutagenesis (SDM) Rat - Point mutation H9C2 CELF1
Product
QuikChange Site-Directed Mutagenesis Kit, 10 Rxn from Agilent Technologies
Manufacturer
Agilent Technologies

Protocol tips

Upstream tips
- XL10-Gold cells are resistant to tetracycline and chloramphenicol. If the mutagenized plasmid contains only the tetR or camR resistance marker, an alternative strain of competent cells must be used
Protocol tips
- Use only the Dpn I enzyme provided; do not substitute with an enzyme from another source.

Publication protocol

Site-directed Mutagenesis
PKC phosphorylation sites were identified using LC-MS/MS on human CELF1 protein expressed in 293T cells. The predicted sites of Ser (28, 52, 178, 179, 241, 300, 302) were mutated to Ala and Thr-173 to Val using site-directed mutagenesis kit (Stratagene 200516) to generate the CELF1M8 construct in p3×FLAG vector.



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Papers

Check out relevant papers found by Labettor's AI that are relevant for performing Site Directed Mutagenesis (SDM) Rat - Point mutation H9C2 CELF1 using QuikChange Site-Directed Mutagenesis Kit, 10 Rxn from Agilent Technologies.

Paper title
Reactivation of Fetal Splicing Programs in Diabetic Hearts Is Mediated by Protein Kinase C Signaling
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Manufacturer protocol

Download the product protocol from Agilent Technologies for QuikChange Site-Directed Mutagenesis Kit, 10 Rxn below.

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