Radius™ 24-Well Cell Migration Assay

Protocol tips

Upstream tips	Protocol tips	Downstream tips
Seed 0.15 - 0.3 x 106 cells/ml.	Incubate plate for 24 hours to allow firm attachment/spreading

Upstream tips
Seed 0.15 - 0.3 x 106 cells/ml.
Protocol tips
Incubate plate for 24 hours to allow firm attachment/spreading

Publication protocol

Cell migration activity of Nox4 or control siRNA transfected SH-SY5Y cells was studied by means of a Radius 24-well cell migration assay (Cell Biolabs) and the following media: HepG2: Eagle’s Minimum Essential Medium, low glucose (1 g/L), 10% FBS, 1% v/v penicillin/streptomycin 10,000 U/mL (Pen/Strep), and non-essential amino acids (M7145 Sigma) and SH-SY5Y: DMEM, high glucose (4.5 g/L), 10% FBS, and 1% v/v Pen/Strep. Cells were seeded into the radius 24-well plate, containing a standardized 0.68 mm hydrogel spot per well, to which cells cannot attach. Cell division (proliferation) was blocked by adding 10 µg/mL mitomycin C. After 24 h, cells reached a density of approximately 80% and subsequently the biocompatible gel was removed to start the migration into the now exposed cell-free area. After 24 h, the cells were fixed and images taken with a Nikon TMS inverted microscope and MetaView software. Area closure was quantitated using the MRI Wound Healing Tool macro for ImageJ ().

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Manufacturer protocol

Download the product protocol from Cell Biolabs for Radius™ 24-Well Cell Migration Assay below.

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