QCM ECMatrix Cell Invasion Assay, 24-well (8 µm), fluorimetric

Protocol tips

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	Cover plate and incubate for 24 hours at 37°C in a CO2 incubator (4- 6% CO2).

Protocol tips
Cover plate and incubate for 24 hours at 37°C in a CO2 incubator (4- 6% CO2).

Publication protocol

Cell invasion was measured using a Cell Invasion Assay kit, according to the manufacturer's instruction. Briefly, the cells in serum-free DMEM were seeded into the upper compartment of the chambers and DMEM, containing 10% FBS, was added into the lower chambers. Following incubation for 24 h at 37°C, the cells on the upper face of the membrane were removed using a cotton swab and t he cells on the lower face were fixed with 16% trichloroacetic acid, stained with 0.1% crystal violet (Life Technologies) and observed under a microscope (IX83; Olympus Corporation).

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Manufacturer protocol

Download the product protocol from Merck Millipore for QCM ECMatrix Cell Invasion Assay, 24-well (8 µm), fluorimetric below.

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