QCM ECMatrix Cell Invasion Assay, 24-well (8 µm), fluorimetric

Cell migration / Invasion cell type - HeLa

Experiment
Cell migration / Invasion cell type - HeLa
Product
QCM ECMatrix Cell Invasion Assay, 24-well (8 µm), fluorimetric from Merck Millipore
Manufacturer
Merck Millipore

Protocol tips

Protocol tips
Starve cells by incubating 18-24 hours prior to assay in appropriate serumfree medium
(DMEM, EMEM, or equivalent

Publication protocol

Cell invasion was measured using a QCMTM 24-well Fluorometric Cell Invasion Assay kit (Millipore), which is composed of 85% type I and 15% type III collagen according to the manufacturer’s instruction. Briefly, cells were seeded onto an invasion chamber insert containing an 8-μm pore size polycarbonate membrane coated with a thin layer of polymerized collagen. Invading cells on the bottom of the insert membrane were visualized and counted under the DM 2500 fluorescence microscope.

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Papers

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Paper title
Human steroid sulfatase induces Wnt/β-catenin signaling and epithelial-mesenchymal transition by upregulating Twist1 and HIF-1α in human prostate and cervical cancer cells
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Manufacturer protocol

Download the product protocol from Merck Millipore for QCM ECMatrix Cell Invasion Assay, 24-well (8 µm), fluorimetric below.

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