96-Well Cell Invasion Assay, Collagen I

Cell migration / Invasion cell type - MDA-MB-231

Experiment
Cell migration / Invasion cell type - MDA-MB-231
Product
96-Well Cell Invasion Assay, Collagen I from Cell Biolabs
Manufacturer
Cell Biolabs

Protocol tips

Upstream tips
Seed 5 × 105 cells
Protocol tips
Cover the plate and transfer to a cell culture incubator for 24 hours
Downstream tips
Read the fluorescence with a fluorescence plate reader at 560 nm.

Publication protocol

Cell invasion was measured using the CytoSelect 96-Well Collagen I Cell Invasion Assay (Cell Biolabs). Cells (5 × 105) were seeded in serum-free media onto polycarbonate membrane inserts (8 μm thick) whose upper surfaces were coated with a uniform layer of dried Bovine Type I Collagen matrix (Cell Biolabs). Inserts were then submerged in media containing 10% fetal bovine serum (FBS), and the cells were cultured for 24 h. Invading cells were stained with cell stain solution, followed by measurement using a Fluoroskan Ascent plate reader (Thermo Scientific) at 560 nm.

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Papers

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Paper title
Periostin suppression induces decorin secretion leading to reduced breast cancer cell motility and invasion
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Manufacturer protocol

Download the product protocol from Cell Biolabs for 96-Well Cell Invasion Assay, Collagen I below.

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