24-Well Cell Invasion Assays, Basement Membrane

Cell migration / Invasion cell type - HCT116

Experiment
Cell migration / Invasion cell type - HCT116
Product
24-Well Cell Invasion Assays, Basement Membrane from Cell Biolabs
Manufacturer
Cell Biolabs

Protocol tips

Protocol tips
Incubate for 48 hours at 37ºC in 5% CO2 atmosphere
Downstream tips
Measure absorbance at 560 nm

Publication protocol

Invasion assays were conducted using the CytoSelect 24-Well Cell Invasion Assay Kit (Cell Biolabs, San Diego, CA, USA). Briefly, 300 μL of HCT116 and HT29 cells (1 × 105cells/ml) in serum-free medium was plated into the CytoSelect basement membrane chamber, respectively, and 500 μL of 10% FBS-containing RPMI was added to the lower well of the invasion plate; both upper and lower chambers contained the 10 uM IWR-1. The chambers were then incubated for 48 h at 37°C in a 5% CO2 atmosphere, non-migratory cells were removed, and migrated cells were stained, dissociated from the membrane, and their absorbance was measured at 560 nm using the microplate reader (model 680; Bio-Rad, Hercules, CA, USA) [65].

Full paper   Login or join for free to view the full paper.

Reviews

24-Well Cell Invasion Assays, Basement Membrane from Cell Biolabs has not yet been reviewed for this experiment

We'd love it if you would be the first to write a review!

Discussion

Start your discussion

Share your thoughts or question with experts in your field

Start a discussion

Papers

Check out relevant papers found by Labettor's AI that are relevant for performing Cell migration / Invasion cell type - HCT116 using 24-Well Cell Invasion Assays, Basement Membrane from Cell Biolabs.

Paper title
IWR-1 inhibits epithelial-mesenchymal transition of colorectal cancer cells through suppressing Wnt/β-catenin signaling as well as survivin expression
Full paper
Login or join for free to view the full paper.

Manufacturer protocol

Download the product protocol from Cell Biolabs for 24-Well Cell Invasion Assays, Basement Membrane below.

Download PDF Download manufacturer protocol

Videos

Check out videos that might be relevant for performing Cell migration / Invasion cell type - HCT116 using 24-Well Cell Invasion Assays, Basement Membrane from Cell Biolabs. Please note that these videos are representative and steps or experiment specific processes must be kept in mind to expect desired results.

We haven't found any additional videos for this experiment / product combination yet.

Become shareholder Discussions About us Contact Privacy Terms