|We used Cultrex 24-well collagen IV cell invasion assay kit (Trevigen, Gaithersburg, MD, USA). Transwell cell culture inserts with an 8-μm pore membrane were coated with collagen type IV. Cells (2 × 105 cells/insert) were seeded onto the upper chamber without FBS, and 500 μL of RPMI 1640 containing 10% FBS was loaded onto the bottom chamber. After 48 h of incubation, the cell dissociation solution was added onto the bottom chamber and incubated 1 h.|
Cultrex® Collagen IV Cell Invasion Assay from Trevigen has not yet been reviewed for this experiment
We'd love it if you would be the first to write a review!
Check out relevant papers found by Labettor's AI that are relevant for performing Cell migration / Invasion cell type - TPC1 using Cultrex® Collagen IV Cell Invasion Assay from Trevigen.
Download the product protocol from Trevigen for Cultrex® Collagen IV Cell Invasion Assay below.Download manufacturer protocol
Check out videos that might be relevant for performing Cell migration / Invasion cell type - TPC1 using Cultrex® Collagen IV Cell Invasion Assay from Trevigen. Please note that these videos are representative and steps or experiment specific processes must be kept in mind to expect desired results.
We haven't found any additional videos for this experiment / product combination yet.