QIAamp DNA Mini Kit

DNA isolation / purification Cells - Immortalized cell lines 3T3

Experiment
DNA isolation / purification Cells - Immortalized cell lines 3T3
Product
QIAamp DNA Mini Kit from Qiagen
Manufacturer
Qiagen

Protocol tips

Downstream tips
- Include RNAse treatment for 15-20 min.
- Ensure EtOH is completely evaporated off of the column prior to elution. Adjust time from 1min to 5 min at 60`C
- Use prewarmed TE buffer to elute the DNA

Publication protocol

Preparation of nucleic acids
Genomic DNA was isolated using the QIAamp DNA Mini Kit (Qiagen). RNA was isolated using the RNeasy Mini Kit (Qiagen) according to the manufacturer’s protocol. Additional treatment with the TURBO DNA-free Kit (Ambion) was performed to eliminate traces of DNA. RNA integrity was assessed by visualization of sharp 28S and 18S rRNA bands on a 1% agarose gel.

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Discussion

Discussion

4 years ago

Author: R. Verma India

DNA isolation column clogged

During centrifugation, the column got clogged and I was unable to continue with the protocol. How can I unclog it?

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Papers

Check out relevant papers found by Labettor's AI that are relevant for performing DNA isolation / purification Cells - Immortalized cell lines 3T3 using QIAamp DNA Mini Kit from Qiagen.

Paper title
Establishment of an Immortalized Skin Keratinocyte Cell Line Derived from the Animal Model
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Manufacturer protocol

Download the product protocol from Qiagen for QIAamp DNA Mini Kit below.

Download PDF Download manufacturer protocol

Videos

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