|The silenced cells were treated with or without the autophagy inhibitors BafA1 (Millipore, 196000) or CQ (Sigma-Aldrich, C6628) and then lysed in RIPA buffer (1% NP40, 50 mM Tris-HCl [pH 7.5], 150 mM NaCl, 0.25% sodium deoxycholate, 1% sodium dodecyl sulfate (SDS), protease inhibitor cocktail and phosphatase inhibitor). The proteins were separated by SDS-PAGE and transferred onto nitrocellulose membranes for immunoblotting.|
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