M-MLV Reverse Transcriptase (200 U/µL)

Protocol tips

Upstream tips	Protocol tips	Downstream tips
	Heat mixture to 65°C for 5 minutes and quick chill on ice and incubate at 37°C for 2 minutes. Incubate 50 minutes at 37°C. Inactivate the reaction by heating at 70°C for 15 minutes

Protocol tips
Heat mixture to 65°C for 5 minutes and quick chill on ice and incubate at 37°C for 2 minutes. Incubate 50 minutes at 37°C. Inactivate the reaction by heating at 70°C for 15 minutes

Publication protocol

Total RNA was extracted from SUP-B15, JM1, and CL-01 cells by Trizol reagent method (Invitrogen). Quality and quantity of RNA was analyzed by NanoDrop ND1000 spectrophotometer. Isolated cellular RNA (2–5 µg) was used for cDNA synthesis by qPCR by adding Oligo-dT primers (Invitrogen) and M-MLV reverse transcriptase (cat. # 28025-013, Invitrogen) as per manufacturer's protocol

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Manufacturer protocol

Download the product protocol from Thermo Fisher Scientific for M-MLV Reverse Transcriptase (200 U/µL) below.

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