Anti-LC3B antibody produced in rabbit

Autophagy assay cell type - SH-SY5Y

Experiment
Autophagy assay cell type - SH-SY5Y
Product
Anti-LC3B antibody produced in rabbit from Sigma-Aldrich
Manufacturer
Sigma-Aldrich

Protocol tips

Protocol tips
Protein lysates were separated by SDS-PAGE and probed with the following antibodies
Downstream tips
Relative levels of protein to ACTB loading control were quantified by densitometry using ImageJ, and normalized to the lane with the highest signal.

Publication protocol

Protein lysates were separated by SDS-PAGE and probed with the following antibodies: SQSTM1/p62 (Abnova, H00008878-M01), BCL2/B-cell CLL/lymphoma 2 (Santa Cruz, sc-492), BECN1/Beclin 1 (Santa Cruz, sc-11427), pro-CASP3/caspase 3 (Santa Cruz, sc-7148), cleaved CASP3/caspase 3 (Cell Signaling, 9661), BAX/BCL2-associated X protein (Santa Cruz, sc-526), LAMP1/Lysosomal-associated membrane protein 1 (Developmental Studies, H4A3), HSPA5/GRP78 (Santa Cruz, sc-1050), ATG7 (Abcam, ab133528), microtubule-associated protein 1 light chain 3 α/LC3A (Sigma, L8918), ACTB/β-actin (Sigma, A5441), and CTSD/cathepsin D (Santa Cruz, sc-6486). Relative levels of protein to ACTB loading control were quantified by densitometry using ImageJ, and normalized to the lane with the highest signal.

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Papers

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Paper title
Inhibition of glycolysis attenuates 4-hydroxynonenal-dependent autophagy and exacerbates apoptosis in differentiated SH-SY5Y neuroblastoma cells
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Manufacturer protocol

Download the product protocol from Sigma-Aldrich for Anti-LC3B antibody produced in rabbit below.

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