Lipofectamine® RNAiMAX Transfection Reagent

siRNA / RNAi /miRNA transfection Human Cells - KG-1 Lipofectamine

Experiment
siRNA / RNAi /miRNA transfection Human Cells - KG-1 Lipofectamine
Product
Lipofectamine® RNAiMAX Transfection Reagent from Thermo Fisher Scientific
Manufacturer
Thermo Fisher Scientific

Protocol tips

Protocol tips
siRNA concentration- 30 nM

Add diluted siRNA to diluted Lipofectamine® RNAiMAX Reagent (1:1 ratio). 

Incubate for 5 minutes at room temperature and add to cells. 

Incubate cells for 3 days at 37°C. 

Publication protocol

siGAPDH-loaded YSK12-MEND and siGAPDH-loaded RNAiMAX were incubated at a siRNA concentration of 30 nM in OPTI-MEM I at 37 °C, 5% CO2, which was the same condition as siRNA transfection experiment. The RNAiMAX preparation was carried out following the manufacturer’s instructions. After 0.5, 1, 2, and 6 h incubation, the diameters were measured with a ZETASIZER Nano.

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Discussion

Discussion

4 years ago

Author: Keith L. Morrison Canada

siRNA/RNAi/miRNA transfection human

I would like to regulate the expression of a gene and in order to do that, I have purchased specific siRNA. After optimizing my transfection protocol and using electroporation I have achieved a 60-70% reduction of the gene of interest. However, I cannot observe a significant reduction of mRNA expression but only a reduction of protein. What might be the problem? Could the problem be in my cell treatment method?

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Papers

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Manufacturer protocol

Download the product protocol from Thermo Fisher Scientific for Lipofectamine® RNAiMAX Transfection Reagent below.

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