Lipofectamine® RNAiMAX Transfection Reagent

siRNA / RNAi /miRNA transfection Human - NK92 cells Lipofectamine

Experiment
siRNA / RNAi /miRNA transfection Human - NK92 cells Lipofectamine
Product
Lipofectamine® RNAiMAX Transfection Reagent from Thermo Fisher Scientific
Manufacturer
Thermo Fisher Scientific

Protocol tips

Protocol tips
Add diluted siRNA to diluted
Lipofectamine® RNAiMAX
Reagent (1:1 ratio).

Incubate for 5 min at RT.

Incubate cells for 24-72 h at 37°C.

Publication protocol

Silencer select validated Bid, p53, and control small interfering RNAs (siRNAs) were purchased from Life Technologies. Subconfluent cells were transfected with siRNA using Lipofectamine RNAiMAX (Life Technologies) in Opti-MEM according to the manufacturer’s instructions.

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Discussion

Discussion

1 year ago

Author: Keith L. Morrison Canada

siRNA/RNAi/miRNA transfection human

I would like to regulate the expression of a gene and in order to do that, I have purchased specific siRNA. After optimizing my transfection protocol and using electroporation I have achieved a 60-70% reduction of the gene of interest. However, I cannot observe a significant reduction of mRNA expression but only a reduction of protein. What might be the problem? Could the problem be in my cell treatment method?

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Papers

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Manufacturer protocol

Download the product protocol from Thermo Fisher Scientific for Lipofectamine® RNAiMAX Transfection Reagent below.

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Videos

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