Reactive Oxygen Species (ROS) Detection Assay Kit

Protocol tips

Upstream tips	Protocol tips	Downstream tips
- 2.5 × 104 cells were seeded for an assay		- The fluorescence intensity was measured using a fluorescence plate reader at Ex/Em. = 488/525 nm.

Upstream tips
- 2.5 × 104 cells were seeded for an assay
Downstream tips
- The fluorescence intensity was measured using a fluorescence plate reader at Ex/Em. = 488/525 nm.

Publication protocol

A ROS assay kit (BioVision) was used to detect the accumulation of intracellular ROS in HepG2 cells. Briefly, cells were treated with different concentrations of DHM (0, 10, 50 or 100 μM) for 6 h, 12 h and 24 h and were subsequently cultured with or without 10 nM H2O2 for 24 h. After removing the medium containing 100 μM DHM, 100 μL of DCFDA mix containing 2.5 × 104 cells was added to each well and incubated for 45 min at 37°C in the dark. Blank wells (with non-stained cells) were also used as a control. The fluorescence intensity was measured using a fluorescence plate reader (EnSpire™ 2300 Multilabel Reader, PE) at Ex/Em. = 488/525 nm.

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Manufacturer protocol

Download the product protocol from Biovision for Reactive Oxygen Species (ROS) Detection Assay Kit below.

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