OxiSelect™ Intracellular ROS Assay Kit (Green Fluorescence)

ROS assay cell type - PC-3 human prostate adenocarcinoma

Experiment
ROS assay cell type - PC-3 human prostate adenocarcinoma
Product
OxiSelect™ Intracellular ROS Assay Kit (Green Fluorescence) from Cell Biolabs
Manufacturer
Cell Biolabs

Protocol tips

Upstream tips
- 2.5 × 105 were seeded.
Protocol tips
- Treated with DCFH-DA for 1 h at 37°C.

- After DCFH-DA treatement, cells were treated with Vitisin A (4 µM) and/or TRAIL (20 ng/ml) for 1 h at 37°C

Publication protocol

PC-3 cells (2.5 × 105) were cultured in a 96-well cell culture plate and then pre-incubated with DCFH-DA for 1 h at 37°C. After incubation, DCFH-DA-loaded cells were treated with Vitisin A (4 µM) and/or TRAIL (20 ng/ml) for 1 h at 37°C. Intracellular levels of ROS were measured by flow cytometry using 2070-diacetyl dichlorofluorescein (DCFDA). ROS production was analyzed using the CellQuest Software with a FACSCalibur flow cytometer. Also, the concentration of DCF was quantified using the OxiSelect Intracellular ROS Assay Kit (Cell Biolabs, CA, USA) and a Fluoroskan Ascent microplate fluorometer (Thomas Scientific, NJ, USA).

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Papers

Check out relevant papers found by Labettor's AI that are relevant for performing ROS assay cell type - PC-3 human prostate adenocarcinoma using OxiSelect™ Intracellular ROS Assay Kit (Green Fluorescence) from Cell Biolabs.

Paper title
Upregulation of Death Receptor 5 and Production of Reactive Oxygen Species Mediate Sensitization of PC-3 Prostate Cancer Cells to TRAIL Induced Apoptosis by Vitisin A.
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Manufacturer protocol

Download the product protocol from Cell Biolabs for OxiSelect™ Intracellular ROS Assay Kit (Green Fluorescence) below.

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