CellROX™ Deep Red Reagent, for oxidative stress detection

Protocol tips

Upstream tips	Protocol tips	Downstream tips
- Cells were pre-treated with different concentrations of Nx.	- Cells were incubated with 5 μm CellROX Deep Red reagent for 30 min at 37°C

Upstream tips
- Cells were pre-treated with different concentrations of Nx.
Protocol tips
- Cells were incubated with 5 μm CellROX Deep Red reagent for 30 min at 37°C

Publication protocol

Following treatment with different concentrations of Nx, indicated cells were incubated with 5 μm CellROX® Deep Red reagent (Invitrogen, Carlsbad, CA) for 30 min at 37°C. ROS levels were measured by flow cytometry according to the manufacturer's protocol and values reported as mean fluorescence intensity.

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Manufacturer protocol

Download the product protocol from Thermo Fisher Scientific for CellROX™ Deep Red Reagent, for oxidative stress detection below.

Download manufacturer protocol

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