EZCell™ Cell Migration/Chemotaxis Assay Kit (24-well, 8 µm)

Protocol tips

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	Cover and incubate the Cell Migration Chamber at 37°C in CO2 incubator for 24 hrs.

Protocol tips
Cover and incubate the Cell Migration Chamber at 37°C in CO2 incubator for 24 hrs.

Publication protocol

A chemotaxis assay was measured using an 8-μ-pore transwell plate (BioVision, Inc., USA) according to the manufacturer’s protocol. Next, 2–3 × 10 serum starved MM cells (LP1 and RPMI-8226), control or ITAG8 overexpressed, suspended in serum free RPMI1640 media were added to the upper chamber in the presence of medium containing 0 or 30 nM recombinant SDF1α (ProSpec, USA) in the lower chamber. After 24 h of incubation, the cells in the lower chamber were counted using Countess II FL (Thermo Fisher Scientific, USA) or the O.D. value at 450 nm was measured using a Versamax microplate reader (Molecular Devices).

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Manufacturer protocol

Download the product protocol from Biovision for EZCell™ Cell Migration/Chemotaxis Assay Kit (24-well, 8 µm) below.

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