In Vitro Angiogenesis Assay Kit

Angiogenesis assay human - HUVEC

Experiment
Angiogenesis assay human - HUVEC
Product
In Vitro Angiogenesis Assay Kit from Merck Millipore
Manufacturer
Merck Millipore

Protocol tips

Protocol tips
Cells are observed by microscopy after 6h of incubation.

Publication protocol

Capillary tube formation was used to assess the angiogenic potential of releasates generated from resting platelets or platelets stimulated with agonists using the Millipore Capillary Tube Formation Assay kit (Billerica, MA) in duplicate. Primary human umbilical vein endothelial cells (HUVECs) were purchased from Lonza (Walkersville, MD) and cultured in EBM media (Lonza) according to their protocol. After a 6-hour incubation of HUVEC cells with platelet releasate, capillary tube formation was quantified. Five fields were imaged (at ×4 and ×20 magnification) with differential-interference-contrast microscopy using a Zeiss Axiovert microscope, and the degree of tubulogenesis was quantified by counting branch points (nodes with 3 or more branches). Independent assays were averaged, and statistical analysis was performed using the Student t test.


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Discussion

Discussion

2 years ago

Author: Sander J. Mikkelsen Denmark

endothelial cells vs HUVEC cells?

What is the difference between endothelial cells and HUVEC cells for angiogenesis assay?

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Papers

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Paper title
Anticoagulation inhibits tumor cell–mediated release of platelet angiogenic proteins and diminishes platelet angiogenic response
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Manufacturer protocol

Download the product protocol from Merck Millipore for In Vitro Angiogenesis Assay Kit below.

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