In Vitro Angiogenesis Assay Kit

Protocol tips

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	- Cells were seeded in ECG medium with or without 100 ng/mL human rIL-9. - After seeding cells on ECMatrix it is incubated for 48h and assayed after 24 and 48h.

Protocol tips
- Cells were seeded in ECG medium with or without 100 ng/mL human rIL-9. - After seeding cells on ECMatrix it is incubated for 48h and assayed after 24 and 48h.

Publication protocol

IL-9-dependent tube formation in HMVEC cells was assayed using the In Vitro Angiogenesis Assay Kit (Millipore, Billerica, MA). Total 2×104 cells were seeded in ECG medium with or without 100 ng/mL human rIL-9 (eBiosciences) and then cells placed on top of ECMatrix gels in 48-well plates and incubated for 48 hours. Tube formation was assayed after 24 and 48 hours.


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