In Vitro Angiogenesis Assay Kit

Protocol tips

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	- Cells are treated with or without aspalatone (10 μM, 20 μM, 50 μM, and 100 μM) and incubated for additional 18 h.

Protocol tips
- Cells are treated with or without aspalatone (10 μM, 20 μM, 50 μM, and 100 μM) and incubated for additional 18 h.

Publication protocol

HAEC tube formation assay was performed by using an in vitro angiogenesis kit from EMD Millipore following manufacturer's instructions. Briefly, 50 μL of diluted ECM matrix solution was added to each well of 96-well plate and incubated at 37°C to allow the matrix to solidify. Growth arrested HAECs were harvested and then seeded carefully without disturbing the solidified matrix at 1 × 104 cells/well in the absence and presence of aspalatone (10 μM, 20 μM, 50 μM, and 100 μM) and incubated for additional 18 h. Photographs were taken and the length of the capillary network was quantitated as described earlier


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Manufacturer protocol

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