Cultrex® In Vitro Angiogenesis Assay Tube Formation Kit

Angiogenesis assay human - PMVEC

Experiment
Angiogenesis assay human - PMVEC
Product
Cultrex® In Vitro Angiogenesis Assay Tube Formation Kit from Trevigen
Manufacturer
Trevigen

Protocol tips

Protocol tips
- Plates are coated with Reduced Growth Factor Basement Membrane Extract and observed by microscopy after 16h.

Publication protocol

In vitro tube formation was assessed using The Cultrex In vitro Angiogenesis Assay Kit following manufacturer's protocol (Trevigen, Gaithersburg, MD). Cells were first counted, then PMVECs in starvation media containing either vehicle (H2O), BMP2 (10 ng/ml), or VEGF (50 ng/ml) were plated into 24-well plates coated with Reduced Growth Factor Basement Membrane Extract (Trevigen). For all experiments performed with WT and Tie2CrePPARγflox/flox mice, representative images of endothelial tube networks were obtained at 16 h under a fluorescence microscope, and the total tube area per field at 10× magnification was quantified in a blinded fashion using Bioquant Image Analysis software (R&M Biometrics, Nashville, TN). Calcein pre-treatment was used to fluorescently label all living murine PMVECs (Trevigen). In experiments performed with human PMVECs, cells were first fixed and stained following the protocol provided by the manufacturer (Trevigen) and the identification of endothelial tube formation was done under a light microscope (10× magnification) from three different fields. The total tube area per field at 10× magnification was quantified using Wimasis Image Analysis software (Wimasis GmbH, Munich, Germany).


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Discussion

Discussion

2 years ago

Author: Sander J. Mikkelsen Denmark

endothelial cells vs HUVEC cells?

What is the difference between endothelial cells and HUVEC cells for angiogenesis assay?

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Papers

Check out relevant papers found by Labettor's AI that are relevant for performing Angiogenesis assay human - PMVEC using Cultrex® In Vitro Angiogenesis Assay Tube Formation Kit from Trevigen.

Paper title
Loss of PPARγ in endothelial cells leads to impaired angiogenesis
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Manufacturer protocol

Download the product protocol from Trevigen for Cultrex® In Vitro Angiogenesis Assay Tube Formation Kit below.

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