In Vitro Angiogenesis Assay Kit

Angiogenesis assay human - ECV304

Experiment
Angiogenesis assay human - ECV304
Product
In Vitro Angiogenesis Assay Kit from Merck Millipore
Manufacturer
Merck Millipore

Protocol tips

Protocol tips
- Incubate at 37C for at 2 hours to allow the matrix solution to solidify.

Publication protocol

Matrigel assay was used to evaluate capillary tube formation activity as described in the protocol of In Vitro Angiogenesis Assay Kit (Millipore Co., USA). Briefly, ECMatrix™ solution was thawed on ice overnight, then mixed with diluent and placed in a 96-well plate at 37° for 2h to allow the matrix solution to solidify. Each group ECV304 was added on the polymerized matrigel at 104 cells per well. After incubation at 37° for 10h, capillary tubes were inspected at a magnification of 400× with an inverted phase contrast microscope. Capillary tubes were defined as endothelial cord formations that were connected at both ends. The number of tubes in six random fields per well was determined


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Discussion

Discussion

2 years ago

Author: Sander J. Mikkelsen Denmark

endothelial cells vs HUVEC cells?

What is the difference between endothelial cells and HUVEC cells for angiogenesis assay?

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Papers

Check out relevant papers found by Labettor's AI that are relevant for performing Angiogenesis assay human - ECV304 using In Vitro Angiogenesis Assay Kit from Merck Millipore.

Paper title
A Microarray Analysis of Angiogenesis Modulation Effect of Xuefu Zhuyu Decoction( ) on Endothelial Cell
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Manufacturer protocol

Download the product protocol from Merck Millipore for In Vitro Angiogenesis Assay Kit below.

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