BD Cycletest™ Plus DNA Kit

Cell cycle assay human - MCF-7

Experiment
Cell cycle assay human - MCF-7
Product
BD Cycletest™ Plus DNA Kit from BD Biosciences
Manufacturer
BD Biosciences

Protocol tips

Upstream tips
- After cells are seeded and treated with vernodalin for 24 hours, cells were fixed with 70% ethanol overnight.

Publication protocol

1×105 cells per ml seeded overnight in 25 cm2 flask were treated with vernodalin for 24 hours. Cells were then fixed with 70% ethanol overnight. Cells were washed twice with PBS and stained with CycleTEST™ PLUS DNA Reagent Kit (BD Biosciences) according to manufacturer’s instructions. Cell cycle distribution of nuclear DNA was determined by flow cytometry (BD Biosciences) by analyzing at least 20,000 cells per sample. The percentage of cells in G1, S and G2 phases were analyzed by Diva software (BD Biosciences).

Full paper   Login or join for free to view the full paper.

Reviews

BD Cycletest™ Plus DNA Kit from BD Biosciences has not yet been reviewed for this experiment

We'd love it if you would be the first to write a review!

Discussion

Start your discussion

Share your thoughts or question with experts in your field

Start a discussion

Papers

Check out relevant papers found by Labettor's AI that are relevant for performing Cell cycle assay human - MCF-7 using BD Cycletest™ Plus DNA Kit from BD Biosciences.

View full paper   Login or join for free to view the full paper.

Manufacturer protocol

Download the product protocol from BD Biosciences for BD Cycletest™ Plus DNA Kit below.

Download PDF Download manufacturer protocol

Videos

Check out videos that might be relevant for performing Cell cycle assay human - MCF-7 using BD Cycletest™ Plus DNA Kit from BD Biosciences. Please note that these videos are representative and steps or experiment specific processes must be kept in mind to expect desired results.

We haven't found any additional videos for this experiment / product combination yet.

Outsource your experiment

Fill out your contact details and receive price quotes in your Inbox

  Outsource experiment
Become shareholder Discussions About us Contact Privacy Terms