BD Cycletest™ Plus DNA Kit

Cell cycle assay human - MCF-7

Experiment
Cell cycle assay human - MCF-7
Product
BD Cycletest™ Plus DNA Kit from BD Biosciences
Manufacturer
BD Biosciences

Protocol tips

Upstream tips
- After cells are seeded and treated with vernodalin for 24 hours, cells were fixed with 70% ethanol overnight.

Publication protocol

1×105 cells per ml seeded overnight in 25 cm2 flask were treated with vernodalin for 24 hours. Cells were then fixed with 70% ethanol overnight. Cells were washed twice with PBS and stained with CycleTEST™ PLUS DNA Reagent Kit (BD Biosciences) according to manufacturer’s instructions. Cell cycle distribution of nuclear DNA was determined by flow cytometry (BD Biosciences) by analyzing at least 20,000 cells per sample. The percentage of cells in G1, S and G2 phases were analyzed by Diva software (BD Biosciences).

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