Muse® Cell Cycle Assay Kit

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	- Cells are incubated with securinine at concentrations of 10.0 and 20.0 μg/ml for 24 h and 48 h. DMSO concentration added to the cells was 0.2% (v/v)

Protocol tips
- Cells are incubated with securinine at concentrations of 10.0 and 20.0 μg/ml for 24 h and 48 h. DMSO concentration added to the cells was 0.2% (v/v)

Publication protocol

HeLa cells were seeded (5x105/well) and incubated with securinine at concentrations of 10.0 and 20.0 μg/ml for 24 h and 48 h. DMSO concentration added to the cells was 0.2% (v/v). After treating, the cells were harvested and stained with reagents of Muse Cell Cycle Assay Kit (Merck Millipore). Quantitative measurement of the percentage of the cells in G0/G1, S, and G2/M phases of cell cycle was done on Muse Cell Analyzer. The experiments were performed in triplicate.


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