|- Cells were were incubated with various concentrations of 1 (0, 25, 50, 75, 100, 150 and 200 µM); 2 (200 µM); 3 (200 µM); 4 (200 µM); 5 (500 µM); a mixture of ManNAc (125 µM) and 5 (500 µM); and ManNAc (10 mM) for five days.|
|- Fixed with 78% aqueous
ethanol and incubated for 24h
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