FITC BrdU Flow Kit

Protocol tips

Upstream tips	Protocol tips	Downstream tips
- Cells were seeded and treated with or without recombinant human CCL2 (50 mg/ml) for 24 hours.

Upstream tips
- Cells were seeded and treated with or without recombinant human CCL2 (50 mg/ml) for 24 hours.

Publication protocol

THP-1 cells grown in 24-well plates with or without recombinant human CCL2 (50 mg/ml) for 24 hours were incubated with bromodeoxyuridine at various time-points as described by the manufacturers (FITC BrdU flow kit, BD Pharmingen, San Diego, CA). THP1 cells were harvested, fixed and labelled with anti-BrdU fluorescein isothiocyanate antibody and 7-AAD to analyze cell cycle status by FCM.

Full paper   Login or join for free to view the full paper.

Reviews

Discussion

Papers

Check out relevant papers found by Labettor's AI that are relevant for performing Cell cycle assay human - THP-1 using FITC BrdU Flow Kit from BD Biosciences.

Manufacturer protocol

Download the product protocol from BD Biosciences for FITC BrdU Flow Kit below.

Download manufacturer protocol

Videos

Check out videos that might be relevant for performing Cell cycle assay human - THP-1 using FITC BrdU Flow Kit from BD Biosciences. Please note that these videos are representative and steps or experiment specific processes must be kept in mind to expect desired results.