FxCycle™ PI/RNase Staining Solution

Protocol tips

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	- Cells were pretreated with incubated with dimethyl sulfoxide, sulindac (30, 60 and 120 µM), atorvastatin (0.1, 0.5, 1 µM) or Sul/Atorva. - Cells were fixed with 70% ethanol overnight.

Protocol tips
- Cells were pretreated with incubated with dimethyl sulfoxide, sulindac (30, 60 and 120 µM), atorvastatin (0.1, 0.5, 1 µM) or Sul/Atorva. - Cells were fixed with 70% ethanol overnight.

Publication protocol

SW480 colon carcinoma cells were incubated with dimethyl sulfoxide, sulindac (30, 60 and 120 µM), atorvastatin (0.1, 0.5, 1 µM) or Sul/Atorva. The doses reflect plasma concentrations in humans.27 28 After 48 hours of treatment, the cells were washed and fixed in 70% ethanol overnight. Cells were rinsed in phosphate-buffered saline, resuspended in 0.5 mL FxCycle PI/RNase Staining Solution (Life Technologies) and incubated at room temperature for 30 min. DNA content was determined using a BD LSR II Flow Cytometer and FlowJo software.

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