Cell Cycle and Apoptosis Analysis Kit

Protocol tips

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	- Cells are fixed for 24h at 4°C

Protocol tips
- Cells are fixed for 24h at 4°C

Publication protocol

Cells in the log phase of growth were collected and fixed with 70% ice-cold ethanol for 24 h at 4°C. The fixed samples were stained using the Cell Cycle and Apoptosis Analysis Kit (Beyotime, China) according to the manufacturer’s instructions. The staining solution contains buffer, PI, and RNase A. The stained samples were analyzed using BD FACSCanto II (BD, USA)


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