Muse® Cell Cycle Assay Kit

Cell cycle assay mouse - RAW 264.7

Experiment
Cell cycle assay mouse - RAW 264.7
Product
Muse® Cell Cycle Assay Kit from Merck Millipore
Manufacturer
Merck Millipore

Protocol tips

Protocol tips
- Cells were pretreated with 100 or 200 µg/ml of EaEAC for 2 h and followed by 1 µg/ml of LPS for 24 h.

- Cells were fixed with 70% ethanol at −20°C for 3 h

Publication protocol

The cell cycle was measured using a Muse™ Cell Cycle kit (cat. no. MCH100106; EMD Millipore, Billerica, MA, USA) according to the manufacturer's instructions. Briefly, RAW264.7 cells were divided into 100 mm2 dishes (2.5×106 cells/dish), then pretreated with 100 or 200 µg/ml of EaEAC for 2 h. Following treatment with 1 µg/ml of LPS for 24 h, cells from subset groups were harvested by centrifugation at 3,000 × g for 5 min, then fixed with 70% ethanol at −20°C for 3 h. The fixed cells were washed with 1X PBS, then added to 200 µl Cell Cycle Reagent. Following incubation at 37°C in a CO2 incubator for 30 min, cell cycles were analyzed using a Muse® Cell Analyzer with the Muse 1.3.1 analysis program (EMD Millipore).

Full paper   Login or join for free to view the full paper.

Reviews

Muse® Cell Cycle Assay Kit from Merck Millipore has not yet been reviewed for this experiment

We'd love it if you would be the first to write a review!

Discussion

Start your discussion

Share your thoughts or question with experts in your field

Start a discussion

Papers

Check out relevant papers found by Labettor's AI that are relevant for performing Cell cycle assay mouse - RAW 264.7 using Muse® Cell Cycle Assay Kit from Merck Millipore.

Paper title
Ethyl acetate extract from exerts anti-inflammatory effects in LPS-stimulated RAW264.7 macrophage cells by regulating COX-2/iNOS, inflammatory cytokine expression, MAP kinase pathways, the cell cycle and anti-oxidant activity
Full paper
Login or join for free to view the full paper.

Manufacturer protocol

Download the product protocol from Merck Millipore for Muse® Cell Cycle Assay Kit below.

Download PDF Download manufacturer protocol

Videos

Check out videos that might be relevant for performing Cell cycle assay mouse - RAW 264.7 using Muse® Cell Cycle Assay Kit from Merck Millipore. Please note that these videos are representative and steps or experiment specific processes must be kept in mind to expect desired results.

We haven't found any additional videos for this experiment / product combination yet.

Become shareholder Discussions About us Contact Privacy Terms