Dual-Light™ Luciferase & β-Galactosidase Reporter Gene Assay System

Protocol tips

Upstream tips	Protocol tips	Downstream tips
	Dilute Galacton-Plus substrate 1:100 in Buffer B. Add 25 μL of Buffer A to each well. Within 10 min, inject 100 μL of Buffer B (containing Galacton-Plus substrate). After a 1-2 sec delay, read the luciferase signal for 0.1-1 sec/well Incubate for 30-60 min at room temperature

Protocol tips
Dilute Galacton-Plus substrate 1:100 in Buffer B. Add 25 μL of Buffer A to each well. Within 10 min, inject 100 μL of Buffer B (containing Galacton-Plus substrate). After a 1-2 sec delay, read the luciferase signal for 0.1-1 sec/well Incubate for 30-60 min at room temperature

Publication protocol

Cells were cultured and transfected in 96-well Greiner plates. The levels of β-galactosidase and luciferase activity were quantified with Dual-Light luciferase and β-galactosidase gene reporter assay system (Invitrogen, T1004) according to manufacturer’s instructions. Fluorescence and luminescence were measured with the EnVision Multilabel plate reader (Perkin Elmer). For each siRNA transfection, at least 3 replicates were obtained.

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Manufacturer protocol

Download the product protocol from Thermo Fisher Scientific for Dual-Light™ Luciferase & β-Galactosidase Reporter Gene Assay System below.

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