Senescence Detection Kit I (histochemical)

Reporter gene assay β-galactosidase substrates - HUVEC

Experiment
Reporter gene assay β-galactosidase substrates - HUVEC
Product
Senescence Detection Kit I (histochemical) from PromoKine
Manufacturer
PromoKine

Protocol tips

Protocol tips
Fix the cells with 0.5 ml of Fixative Solution for 10-15 minutes at room temperature.

Add 0.5 ml of the Staining Solution Mix.

Cover the plate. Incubate overnight at 37°C

Publication protocol


SA-ß-Gal activity was performed using a Promokine Senescence detection kit (PK-CA577-K320, PromoCell) according to the manufacturer’s instructions. Percentage of SA ß-gal positive cells was counted in 10 randomly selected microscopic fields (magnification ×20; 400–600 cells).

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Papers

Check out relevant papers found by Labettor's AI that are relevant for performing Reporter gene assay β-galactosidase substrates - HUVEC using Senescence Detection Kit I (histochemical) from PromoKine.

Paper title
Biogenesis of Pro-senescent Microparticles by Endothelial Colony Forming Cells from Premature Neonates is driven by SIRT1-Dependent Epigenetic Regulation of MKK6
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Manufacturer protocol

Download the product protocol from PromoKine for Senescence Detection Kit I (histochemical) below.

Download PDF Download manufacturer protocol

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