Senescence β-Galactosidase Staining Kit - Cell Signaling

Reporter gene assay β-galactosidase substrates - H1299

Experiment
Reporter gene assay β-galactosidase substrates - H1299
Product
Senescence β-Galactosidase Staining Kit - Cell Signaling from Cell Signaling Technology
Manufacturer
Cell Signaling Technology

Protocol tips

Protocol tips
Treat cells with kit after 96 hrs of incubation.

Add 1 ml of the β-Galactosidase Staining Solution and incubate the plate at 37°C at least overnight in a dry incubator (no CO2)

Publication protocol

To investigate senescence, the SA-β-gal activity in cultured H1299 cells was detected using the Senescence β-Galactosidase Staining kit (Cell Signaling) according to the manufacturer's recommendations. Briefly, H1299 cells were transfected with indicated plasmids. Four days after transfection, Senescence β-Galactosidase Staining was performed.

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Papers

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Paper title
IκB Kinase β (IKKβ) Inhibits p63 Isoform γ (TAp63γ) Transcriptional Activity
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Manufacturer protocol

Download the product protocol from Cell Signaling Technology for Senescence β-Galactosidase Staining Kit - Cell Signaling below.

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