Senescence β-Galactosidase Staining Kit - Beyotime

Reporter gene assay β-galactosidase substrates - HepG2

Experiment
Reporter gene assay β-galactosidase substrates - HepG2
Product
Senescence β-Galactosidase Staining Kit - Beyotime from Beyotime
Manufacturer
Beyotime

Protocol tips

Protocol tips
Add 1 ml of the β-Galactosidase Staining Solution

Incubate overnight at 37 ° C without Co2

Publication protocol

Senescent cells were analyzed using a SA -gal staining kit (Beyotime Inc., Nantong, China) according to the manufacturer's instructions. The percentage of SA--gal positive cells was calculated by counting the cells in 5 random fields (at least 100 cells) using bright-field microscopy, according to the method reported by te Poele et al [].

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Papers

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Paper title
miR-34a induces cellular senescence via modulation of telomerase activity in human hepatocellular carcinoma by targeting FoxM1/c-Myc pathway
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Manufacturer protocol

Download the product protocol from Beyotime for Senescence β-Galactosidase Staining Kit - Beyotime below.

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