Senescence β-Galactosidase Staining Kit - Cell Signaling

Reporter gene assay β-galactosidase substrates - Huh7

Experiment
Reporter gene assay β-galactosidase substrates - Huh7
Product
Senescence β-Galactosidase Staining Kit - Cell Signaling from Cell Signaling Technology
Manufacturer
Cell Signaling Technology

Protocol tips

Protocol tips
Fix cells with Fixative Solution for 15 minutes at room temperature,

Incubate overnight (cells) at 37 ° C without Co2 and 48 hours (frozen sections)

Publication protocol

β-galactosidase staining was performed using Senescence β-Galactosidase Staining Kit (Cat#9860, Cell Signaling Technology)13, following the manufacturers’ instructions. Briefly, HCC cells grown in 6-well plates or frozen sections from xenograft tissues were fixed with Fixative Solution for 15 minutes at room temperature, and then incubated with β-Galactosidase Staining Solution at 37 °C in a dry incubator without CO2 overnight (cell lines) or 48 hours (frozen sections). Slides were counterstained with Eosin. Cells or slides were examined under a Nikon microscope for the development of blue color.

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Papers

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Paper title
Utility of the dual-specificity protein kinase TTK as a therapeutic target for intrahepatic spread of liver cancer
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Manufacturer protocol

Download the product protocol from Cell Signaling Technology for Senescence β-Galactosidase Staining Kit - Cell Signaling below.

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