Senescence β-Galactosidase Staining Kit - Cell Signaling

Reporter gene assay β-galactosidase substrates - mouse cardiac fibroblasts

Experiment
Reporter gene assay β-galactosidase substrates - mouse cardiac fibroblasts
Product
Senescence β-Galactosidase Staining Kit - Cell Signaling from Cell Signaling Technology
Manufacturer
Cell Signaling Technology

Protocol tips

Protocol tips
Fix cells for 5 minutes in 3.7% formaldehyde..

Add 1 ml of the β-Galactosidase Staining Solution and incubate the plate at 37°C at least overnight in a dry incubator (no CO2)

Publication protocol

Senescence-associated β-gal staining was performed using the senescence-galactosidase staining kit (Cell Signaling Technology) according to the manufacturer's instructions. Briefly, cells were fixed for 5 minutes in 3.7% formaldehyde, washed with PBS and stained in β-galactosidase staining solution at 37°C until the intensity of staining was visible in either experimental or control plates. The number of positive cells was counted under the light microscope.

For tissue staining, mice were killed using carbon dioxide followed by isolation of the hearts. The heart tissues of wild-type and transgenic mice were excised, rapidly frozen in liquid nitrogen and mounted in OCT. Sections (4 µm) were cut, mounted onto glass slides, fixed in 2% formalin in PBS for 1 minute at room temperature, and immersed in β-gal staining solution at 37°C for 24 hours. The samples were counterstained with eosin and viewed under brightfield at 40× magnification.

Full paper   Login or join for free to view the full paper.

Reviews

Senescence β-Galactosidase Staining Kit - Cell Signaling from Cell Signaling Technology has not yet been reviewed for this experiment

We'd love it if you would be the first to write a review!

Discussion

Start your discussion

Share your thoughts or question with experts in your field

Start a discussion

Papers

Check out relevant papers found by Labettor's AI that are relevant for performing Reporter gene assay β-galactosidase substrates - mouse cardiac fibroblasts using Senescence β-Galactosidase Staining Kit - Cell Signaling from Cell Signaling Technology.

Paper title
The microRNA miR-17-3p inhibits mouse cardiac fibroblast senescence by targeting Par4.
Full paper
Login or join for free to view the full paper.

Manufacturer protocol

Download the product protocol from Cell Signaling Technology for Senescence β-Galactosidase Staining Kit - Cell Signaling below.

Download PDF Download manufacturer protocol

Videos

Check out videos that might be relevant for performing Reporter gene assay β-galactosidase substrates - mouse cardiac fibroblasts using Senescence β-Galactosidase Staining Kit - Cell Signaling from Cell Signaling Technology. Please note that these videos are representative and steps or experiment specific processes must be kept in mind to expect desired results.

We haven't found any additional videos for this experiment / product combination yet.

Become shareholder Discussions About us Contact Privacy Terms