β-Galactosidase Reporter Gene Staining Kit

Reporter gene assay β-galactosidase substrates - rat mesenchymal stem cells (MSCs)

Experiment
Reporter gene assay β-galactosidase substrates - rat mesenchymal stem cells (MSCs)
Product
β-Galactosidase Reporter Gene Staining Kit from Sigma-Aldrich
Manufacturer
Sigma-Aldrich

Protocol tips

Upstream tips
Seed 4×10^4 cells
Protocol tips
After 48h , fix cells for 10 min

Add β-Galactosidase Reporter Gene Stain and incubate for 12 h at 37°C.
Downstream tips
Read OD at 420 nm

Publication protocol

The cells (4×104) were seeded into 6-well plates. After 48 h, the cells were washed twice with PBS and fixed for 10 min. After removing the fixative, the cells were washed twice with PBS and stained with the staining solution provided by the β-Galactosidase Reporter Gene Staining kit (Sigma-Aldrich) for 12 h at 37°C, and the percentage of β-galactosidase-positive cells was then determined by randomly counting 5 fields on a phase contrast microscope (Olympus Corp.).

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Papers

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Paper title
Expression of human telomerase reverse transcriptase mediates the senescence of mesenchymal stem cells through the PI3K/AKT signaling pathway.
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Manufacturer protocol

Download the product protocol from Sigma-Aldrich for β-Galactosidase Reporter Gene Staining Kit below.

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