Beta-Galactosidase Assay

Protocol tips

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	Add 2 mL of 1X Fixing Solution. Incubate at room temperature for 5 minutes. Wash cells and add Cell Staining Working Solution. Incubate the cells at 37ºC protected from light for 1 hr to overnight.

Protocol tips
Add 2 mL of 1X Fixing Solution. Incubate at room temperature for 5 minutes. Wash cells and add Cell Staining Working Solution. Incubate the cells at 37ºC protected from light for 1 hr to overnight.

Publication protocol

The Ins-1 rat insulinoma cell clone 832/13, provided by C. Wollheim, was maintained in RPMI 1640 medium as previously described (11,12). Cell metabolic activity was tested with the 0.5% tetrazolium salt solution 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) or WST-1 (Roche) according to the manufacturer’s instructions. Cell death was quantified by trypan blue exclusion or lactate dehydrogenase (LDH) release in the medium (Roche). Cellular senescence was quantified with the β-galactosidase assay kit (Cell Biolabs). Adenovirus-expressing p21 (Adp21) (rat) and adenovirus-containing green fluorescent protein (AdGFP) were purchased from Vector Biolabs (Philadelphia, PA). Rac1 activity was measured with G-LISA Rac1 activation assay (Cytoskeleton, Denver, CO).

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Manufacturer protocol

Download the product protocol from Cell Biolabs for Beta-Galactosidase Assay below.

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