jetPEI® DNA transfection, HTS application

DNA transfection Mammalian cells - Primary cells Human astrocytes

Experiment
DNA transfection Mammalian cells - Primary cells Human astrocytes
Product
jetPEI® DNA transfection, HTS application from Polyplus transfections
Manufacturer
Polyplus transfections

Protocol tips

Protocol tips
Do not add antibiotics to the Transfection Medium

Publication protocol

ERK overexpression
Cells were transfected with human CMV6-XL4 MAPK (Origene, Rockville, MD, USA) or with the plasmid alone by the PEI method (JetPEI, Polyplus transfection, []). Two days after transfection, astrocytes were trypsinized and seeded in medium containing 200 μg/ml ampicillin. MAPK overexpression was verified by western blot, as described below.

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Papers

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Paper title
Trazodone regulates neurotrophic/growth factors, mitogen-activated protein kinases and lactate release in human primary astrocytes
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Manufacturer protocol

Download the product protocol from Polyplus transfections for jetPEI® DNA transfection, HTS application below.

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