Lipofectamine® 2000 Transfection Reagent

DNA transfection Mammalian cells - Primary cells Human osteoblasts

Experiment
DNA transfection Mammalian cells - Primary cells Human osteoblasts
Product
Lipofectamine® 2000 Transfection Reagent from Thermo Fisher Scientific
Manufacturer
Thermo Fisher Scientific

Protocol tips

Protocol tips
Do not add antibiotics to the Transfection Medium

Publication protocol

ERα overexpression
For transfection experiments, the cells were plated in 6-well plates and maintained in phenol-red free DMEM containing 10% charcoal stripped FCS, and were transiently transfected with 2.5 μg of pSG5HEO (the wild-type human ERα expression vector) using LipofectamineTM 2000 transfection reagent (Thermo Fisher Scientific, Waltham, USA) in the presence of 10 nM 17-β-estradiol (Sigma-Aldrich). 48 h post transfection, cells were harvested and lysed for Western blot analysis.

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Papers

Check out relevant papers found by Labettor's AI that are relevant for performing DNA transfection Mammalian cells - Primary cells Human osteoblasts using Lipofectamine® 2000 Transfection Reagent from Thermo Fisher Scientific.

Paper title
The expression of cystathionine gamma-lyase is regulated by estrogen receptor alpha in human osteoblasts
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Manufacturer protocol

Download the product protocol from Thermo Fisher Scientific for Lipofectamine® 2000 Transfection Reagent below.

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