Viromer® RED

DNA transfection Mammalian cells - Primary cells Rat schwann cells

Experiment
DNA transfection Mammalian cells - Primary cells Rat schwann cells
Product
Viromer® RED from Lipocalyx GmbH
Manufacturer
Lipocalyx GmbH

Protocol tips

Upstream tips
For adherent cells, you can target up to 80% confluency at the time of analysis
Protocol tips
Always add Buffer RED to Viromer® RED, not vice versa!

Publication protocol

2.4. Transfection
Cells were seeded on either 24- or 96-well cell culture treated plates at a density to ensure ~80% confluent cultures at 24 hr after seeding. Typically 80,000 cells/cm2 surface area of the culture dish were seeded in cell-specific medium. Transfection using Lipofectamine 3000 was performed according to the manufacturer's protocol with a DNA to Lipofectamine ratio of 1 : 3 w/v. A transfection enhancer, the 3000 enhancer reagent (1 : 2, DNA : Reagent, w/v), was used along with the Lipofectamine 3000 transfection reagent for all transfections. Typically 100 ng and 500 ng of plasmid DNA were transferred to each well of the 96-well plate and 24-well plates, respectively. The standard complexation protocol was employed for Viromer RED according to the manufacturer's instructions. Briefly, Viromer RED was diluted (1 : 24 v/v) and the plasmid DNA was diluted independently to 18 ng/μL in the provided dilution buffer E. A 22 μL volume of diluted DNA was added to 4 μL of diluted Viromer RED and the mixture then was allowed to stay at room temperature for 15 min. For transfection, 100 ng of plasmid DNA, Viromer RED mixture, was added to each well of the 96-well plate by dispensing 6.7 μL per well. In specified experiments, 6 hr before transfection, the media was changed to either DMEM with antibiotics and without serum or DMEM with antibiotics and with serum. For quantitative evaluation of protease activity (as a measure of cell viability), luminometry, or fluorescence microscopy, cells were used 24 hr after transfection.



Full paper   Login or join for free to view the full paper.

Reviews

Viromer® RED from Lipocalyx GmbH has not yet been reviewed for this experiment

We'd love it if you would be the first to write a review!

Discussion

Start your discussion

Share your thoughts or question with experts in your field

Start a discussion

Papers

Check out relevant papers found by Labettor's AI that are relevant for performing DNA transfection Mammalian cells - Primary cells Rat schwann cells using Viromer® RED from Lipocalyx GmbH.

View full paper   Login or join for free to view the full paper.

Manufacturer protocol

Download the product protocol from Lipocalyx GmbH for Viromer® RED below.

Download PDF Download manufacturer protocol

Videos

Check out videos that might be relevant for performing DNA transfection Mammalian cells - Primary cells Rat schwann cells using Viromer® RED from Lipocalyx GmbH. Please note that these videos are representative and steps or experiment specific processes must be kept in mind to expect desired results.

We haven't found any additional videos for this experiment / product combination yet.

Become shareholder Discussions About us Contact Privacy Terms