FuGENE® HD Transfection Reagent

DNA transfection Mammalian cells - Primary cells Rat mesangial cells

Experiment
DNA transfection Mammalian cells - Primary cells Rat mesangial cells
Product
FuGENE® HD Transfection Reagent from Promega
Manufacturer
Promega

Protocol tips

Upstream tips
Always bring FuGENE HD to room temperature and mix well prior to use.

Publication protocol

Transfection.
Mesangial cells were transfected with plasmid vectors or siRNAs targeted against Erk5 using FuGENE HD essentially as described previously (14, 15). For siRNA transfection, scrambled RNA was used as control.

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Papers

Check out relevant papers found by Labettor's AI that are relevant for performing DNA transfection Mammalian cells - Primary cells Rat mesangial cells using FuGENE® HD Transfection Reagent from Promega.

Paper title
A positive feedback loop involving Erk5 and Akt turns on mesangial cell proliferation in response to PDGF
Full paper
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Manufacturer protocol

Download the product protocol from Promega for FuGENE® HD Transfection Reagent below.

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Videos

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