FuGENE® HD Transfection Reagent

DNA transfection Mammalian cells - Immortalized cell lines PC12

Experiment
DNA transfection Mammalian cells - Immortalized cell lines PC12
Product
FuGENE® HD Transfection Reagent from Promega
Manufacturer
Promega

Protocol tips

Upstream tips
Always bring FuGENE HD to room temperature and mix well prior to use.

Publication protocol

Transfection:
Cells at 50% confluency were transiently transfected using FuGENE HD Transfection Reagent (Roche) following the instruction of the manufacturer. Full length human cDNA subcloned into pCMV6-XL5 was purchased from Origene. Plasmid DNA was first diluted in OPTI-MEM I (Gibco-BRL, USA) and then mixed with FuGENE HD Transfection Reagent. Following a 20-minute incubation period at room temperature, the DNA-FuGENE HD Transfection Reagent mixture was added to the antibiotic-free transfection medium (DMEM containing glutamine). The cells were maintained in a 37°C incubator with a saturated humidity air atmosphere containing 5% CO. The medium was replaced with normal culture medium 6 hours later. Cells were used for further experiments 48 hours after transfection.

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Papers

Check out relevant papers found by Labettor's AI that are relevant for performing DNA transfection Mammalian cells - Immortalized cell lines PC12 using FuGENE® HD Transfection Reagent from Promega.

Paper title
Role of Alpha-Synuclein Protein Levels in Mitochondrial Morphology and Cell Survival in Cell Lines
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Manufacturer protocol

Download the product protocol from Promega for FuGENE® HD Transfection Reagent below.

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