FuGENE® HD Transfection Reagent

Protocol tips

Upstream tips	Protocol tips	Downstream tips
Always bring FuGENE HD to room temperature and mix well prior to use.

Upstream tips
Always bring FuGENE HD to room temperature and mix well prior to use.

Publication protocol

Transfection:
Cells at 50% confluency were transiently transfected using FuGENE HD Transfection Reagent (Roche) following the instruction of the manufacturer. Full length human cDNA subcloned into pCMV6-XL5 was purchased from Origene. Plasmid DNA was first diluted in OPTI-MEM I (Gibco-BRL, USA) and then mixed with FuGENE HD Transfection Reagent. Following a 20-minute incubation period at room temperature, the DNA-FuGENE HD Transfection Reagent mixture was added to the antibiotic-free transfection medium (DMEM containing glutamine). The cells were maintained in a 37°C incubator with a saturated humidity air atmosphere containing 5% CO. The medium was replaced with normal culture medium 6 hours later. Cells were used for further experiments 48 hours after transfection.

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Papers

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Manufacturer protocol

Download the product protocol from Promega for FuGENE® HD Transfection Reagent below.

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