LIVE/DEAD™ Viability/Cytotoxicity Kit, for mammalian cells

Protocol tips

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	Add ethidium-calcein mixture, and incubate for 30 min in a 37 °C incubator

Protocol tips
Add ethidium-calcein mixture, and incubate for 30 min in a 37 °C incubator

Publication protocol

Viability of HepG2 cells cultured encapsulated in GelMAhigh and GelMAlow was determined by calcein-ethidium homodimer Live-Dead assay (Invitrogen) of construct on day 3 of culture using manufacturer’s protocol.

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Manufacturer protocol

Download the product protocol from Thermo Fisher Scientific for LIVE/DEAD™ Viability/Cytotoxicity Kit, for mammalian cells below.

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