LIVE/DEAD™ Viability/Cytotoxicity Kit, for mammalian cells

Protocol tips

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	Add 1.2 μL of 2 mM ethidium homodimer-1 (EthD-1) and 0.3 μL of 4 mM calcein AM. Incubated for 30 min with 5% CO2 at 37 °C

Protocol tips
Add 1.2 μL of 2 mM ethidium homodimer-1 (EthD-1) and 0.3 μL of 4 mM calcein AM. Incubated for 30 min with 5% CO2 at 37 °C

Publication protocol

In vitro cell viability and adhesions of the HA-BDDGE patterned gel were observed with MC3T3 cells in the 24-well culture plate. Live & dead viability/cytotoxicity kit for mammalian cells was prepared according to the protocol suggested by the vendor (Invitrogen, Carlsbad, CA, USA). The 1.2 μL of 2 mM ethidium homodimer-1 (EthD-1) and 0.3 μL of 4 mM calcein AM were added into 600 μL PBS and used for live and dead assay. The solution was put in the well plate and incubated for 30 min with 5% CO2 at 37 °C. The images of the MC3T3 cells on the HA-BDDGE patterned gel were captured by a fluorescence microscope (Leica DMLB, Wetzlar, Germany).

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