Live and Dead Cell Assay (Abcam)

Protocol tips

Upstream tips	Protocol tips	Downstream tips
Cells were stained after 24 h of the culture	Incubate cells for 10min at room temperature in the dark.

Upstream tips
Cells were stained after 24 h of the culture
Protocol tips
Incubate cells for 10min at room temperature in the dark.

Publication protocol

An osteoblast viability assay was performed using the PrestoBlue Cell Viability Reagent (Molecular Probes; Invitrogen) according to the manufacturer's protocol. The cells in suspension were seeded at 1 × 105 cells/well in a 96-well microtiter plate. After 24 h, the cells were washed and incubated with the PrestoBlue reagent for 10 min. The changes in cell viability were detected using absorbance spectroscopy at a wavelength of 570 nm. For further analysis of cell viability, another set of the cells was subjected for an apoptosis test by staining them at 24 h of the culture (LIVE and DEAD Cell Assay Kit ab115347; Abcam), by which live cells were detected with green fluorescence and dead with red through a Biozero BZ-8000 microscope (Keyence), and the numbers of live/dead cells were quantified.

Full paper   Login or join for free to view the full paper.

Reviews

Discussion

Papers

Check out relevant papers found by Labettor's AI that are relevant for performing Live / Dead assay mammalian cells - MC3T3 using Live and Dead Cell Assay (Abcam) from Abcam.

Manufacturer protocol

Download the product protocol from Abcam for Live and Dead Cell Assay (Abcam) below.

Download manufacturer protocol

Videos

Check out videos that might be relevant for performing Live / Dead assay mammalian cells - MC3T3 using Live and Dead Cell Assay (Abcam) from Abcam. Please note that these videos are representative and steps or experiment specific processes must be kept in mind to expect desired results.