LIVE/DEAD™ Viability/Cytotoxicity Kit, for mammalian cells

Live / Dead assay mammalian cells - C2C12

Experiment
Live / Dead assay mammalian cells - C2C12
Product
LIVE/DEAD™ Viability/Cytotoxicity Kit, for mammalian cells from Thermo Fisher Scientific
Manufacturer
Thermo Fisher Scientific

Protocol tips

Protocol tips
Add ethidium-calcein mixture, and incubate for 30-45 min at RT.

A shorter incubation time may be used if the dye concentrations or incubation temperature are increased.

Publication protocol

Neonatal cardiomyocytes, ESCs, and noncardiomyocytes including MEFs, HepG2, HEK293, peripheral lymphatic cells, C2C12, skeletal myotubes, and fetal neurons were exposed to glucose-free DMEM (Invitrogen) supplemented with or without lactate (Wako). Cell viabilities were determined by the LIVE/DEAD Viability/Cytotoxicity Assay Kit (Invitrogen) based on the simultaneous determination of live and dead cells with the calcein AM and ethidium homodimer-1 probes. Fluorescence imaging of the cells (live cells were labeled green, whereas the nuclei of dead cells were labeled red) was performed with fluorescence microscopy (IX70 microscope; Olympus) equipped with a color charge-coupled device camera (CS220; Olympus). The green-labeled live area was measured using Image J. Relative cell viabilities were calculated in percentages, compared with those before treatment.

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Papers

Check out relevant papers found by Labettor's AI that are relevant for performing Live / Dead assay mammalian cells - C2C12 using LIVE/DEAD™ Viability/Cytotoxicity Kit, for mammalian cells from Thermo Fisher Scientific.

Paper title
Distinct metabolic flow enables large-scale purification of mouse and human pluripotent stem cell-derived cardiomyocytes.
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Manufacturer protocol

Download the product protocol from Thermo Fisher Scientific for LIVE/DEAD™ Viability/Cytotoxicity Kit, for mammalian cells below.

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