|siRNA concentration-50–100 nM final concentration
Mix siRNA:reagent mixture and incubate at room temperature for 15–30 minutes.
Add mixture to cells and incubate for 24hours.
TransIT-TKO Transfection Reagent from Mirus has not yet been reviewed for this experiment
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1 year ago
Author: Keith L. Morrison
I would like to regulate the expression of a gene and in order to do that, I have purchased specific siRNA. After optimizing my transfection protocol and using electroporation I have achieved a 60-70% reduction of the gene of interest. However, I cannot observe a significant reduction of mRNA expression but only a reduction of protein. What might be the problem? Could the problem be in my cell treatment method?
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