TransIT-TKO Transfection Reagent

siRNA / RNAi /miRNA transfection Human Cells - Primary splenocytes Polymer / lipid

Experiment
siRNA / RNAi /miRNA transfection Human Cells - Primary splenocytes Polymer / lipid
Product
TransIT-TKO Transfection Reagent from Mirus
Manufacturer
Mirus

Protocol tips

Protocol tips
siRNA concentration-50–100 nM final concentration

Mix siRNA:reagent mixture and incubate at room temperature for 15–30 minutes.

Add mixture to cells and incubate for 24hours.

Publication protocol

siAPC-C (gacgttgcgagaagttgga), siAPC729 (gaggtcatctcagaacaag), siAPCL413 (ggtgtttcctgctgaatga), siAPCL5100 (ggcgccaattcaattgtca), siHIF1α [46], siHIF2α [46] or siGFP (aagctacctgttccatggcca) (50–100 nM final concentration) were transfected into cells overnight using 1 µl Transit TKO (MoBiTec, Göttingen, Germany) per µl siRNA (20 µM). The sequences show only the coding strand.

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Reviews

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Discussion

Discussion

4 years ago

Author: Keith L. Morrison Canada

siRNA/RNAi/miRNA transfection human

I would like to regulate the expression of a gene and in order to do that, I have purchased specific siRNA. After optimizing my transfection protocol and using electroporation I have achieved a 60-70% reduction of the gene of interest. However, I cannot observe a significant reduction of mRNA expression but only a reduction of protein. What might be the problem? Could the problem be in my cell treatment method?

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Papers

Check out relevant papers found by Labettor's AI that are relevant for performing siRNA / RNAi /miRNA transfection Human Cells - Primary splenocytes Polymer / lipid using TransIT-TKO Transfection Reagent from Mirus.

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Manufacturer protocol

Download the product protocol from Mirus for TransIT-TKO Transfection Reagent below.

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Videos

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